AFMAN40-401 — The Care And Use Of Laboratory Animals In Dod Programs

AFMAN40-401 governs the care and use of animals in Department of Defense research, development, test, evaluation, and training programs.

Search AFMAN40-401

  • Publication number: AFMAN40-401
  • Title: THE CARE AND USE OF LABORATORY ANIMALS IN DOD PROGRAMS
  • Date: 2022-10-21

View AFMAN40-401 on armypubs.army.mil


It establishes procedures, responsibilities, and policies for animal care and use in DoD-conducted and supported RDT&E or training. The publication addresses oversight offices, IACUCs, training protocols, animal facilities, overseas programs, prohibited uses, and complaints.

Topics covered

  • Animal care and use
  • DoD RDT&E and training programs
  • Institutional Animal Care and Use Committees
  • Animal use oversight
  • Training protocols
  • Animal housing facilities
  • Prohibited animal use
  • Animal programs outside the United States

Questions and answers

What approval is required for DoD animal training protocols?

Ensure that required training protocols are submitted in the DoD standard animal use protocol format or in an alternate format approved by USD(R&E) and are reviewed and approved by a duly constituted IACUC. (paragraph 9)

When must animal facilities and equipment be approved?

Ensure animal housing facilities, enclosures, and equipment are inspected and approved by a DoD veterinarian before receiving animals. (paragraph 9)

What standards apply to DoD animal activities outside the United States?

Activities subject to this publication conducted by the DoD outside the United States will comply with References (d) through (aa), as well as, the host nation requirements for the acquisition, transportation, housing, control, maintenance, handling, treatment, care, welfare, use, and final disposition of animals. (paragraph 1-4)

How often must DoD facilities receive compliance inspections?

To ensure a compliance inspection is conducted at each DoD facility at least every three years by the Component oversight office. (paragraph 0)

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